For laboratory research use only. Not for human or veterinary consumption.

TB-500 Vial 10 mg Lot TB5-10-005: Certificate of Analysis (COA)

Peptira · tested Apr 16, 2026 by Freedom Diagnostics · Most recent COA

HPLC purity
99.25%
Identity (LC-MS)
Confirmed
Test date
Apr 16, 2026

This page summarizes the third-party lab report for lot TB5-10-005 of TB-500 Vial 10 mg sold by Peptira. The lot number on this page should match the lot printed on the vial label. Don't rely on this summary alone: use the button to open the lab's own record and compare the lot, date and results.

Lot-matchedResults are tied to lot TB5-10-005, not to the product in general.
Outside labTested by Freedom Diagnostics, not by the vendor or by us.
Checkable at the sourceSearch Code links to the lab's own record.
Original documentThe vendor's copy of the COA is shown below, unedited.

TB-500 Vial 10 mg lot TB5-10-005 test results, explained

Every result printed on this certificate, as reported by Freedom Diagnostics, with a plain-English note on what the test measures. "Pass" and "specification" wording comes from the certificate; where the lab prints no pass/fail, we say so.

TestResult on the certificateWhat it measuresThis lot
HPLC purity99.25% · Per-vial HPLC purity: 99.25%, 99.31%, 99.25%HPLC separates the sample into its components; purity is the share of the detected signal that sits in the main peak. On its own it doesn't show which molecule that peak is. That's what the identity test is for.No pass/fail printed; reported value at or above 98%
Identity (LC-MS)Confirmed · compound named: Thymosin Beta-4Mass spectrometry measures the molecular mass of what's in the vial and compares it with the declared compound. "Confirmed" means the lab found the expected compound. Expected average molecular weight of TB-500 (thymosin beta-4) (PubChem): 889.0 g/mol. The observed mass appears in the certificate's chart and isn't transcribed here.Reported as matching the declared compound
Quantity (measured content)per vial: 10.75 mg, 11.27 mg, 11.37 mgHow much peptide the lab measured in the vial, compared with the amount printed on the label.Reported
AppearanceWhite Lyophilized PowderWhat the lab recorded before testing: the colour and physical form of the material.Recorded
Endotoxin (LAL assay (USP <85>))Pass (LAL, USP <85>)Bacterial endotoxins are fragments of bacterial cell walls. The LAL assay measures them in endotoxin units (EU/mL); a pass means the result was under the lab's limit.Pass (as reported by the lab)

Lot and report details

Lot / batch numberTB5-10-005
Product (as labeled by vendor)TB-500 Vial 10 mg
Labeled vial size10 mg
Testing laboratoryFreedom Diagnostics
Sample received by labApr 15, 2026
Report dateApr 16, 2026
Method (as stated)HPLC with UV detection coupled with mass spectrometry (LC-MS)
Search CodePept2604150109
Lab-record checkOn Oct 7, 2026 the vendor's PDF was byte-for-byte identical to the copy hosted on the lab's site.
VendorPeptira
VerifyOpen Freedom Diagnostics's record ↗

Data notes: Lab received 04/15/2026, reported 04/16/2026 (MM/DD/YYYY).

The certificate itself

Page 1 of the certificate of analysis for TB-500 Vial 10 mg lot TB5-10-005 (Freedom Diagnostics)
Page 1 of the certificate (Peptira (vendor's copy)), saved here as an image so the page stays fast. Open the original PDF ↗ · Source ↗

About Freedom Diagnostics

A peptide testing lab in Franklin, Tennessee (per the lab's website) that publishes its certificates in a searchable online database.

Accreditation: No accreditation is printed on these certificates, and we found no published ISO/IEC 17025 accreditation for the lab (checked Oct 7, 2026).

Tests: On the certificates here: HPLC purity with LC-MS identity, net content and, where ordered, endotoxin (LAL), a microbial PCR screen and other screens.

How to verify: Search the Search Code on freedomdiagnosticstesting.com. The lab hosts its own PDF copy of each certificate, which should match the vendor's copy.

Open Freedom Diagnostics's record ↗

Lab info from: freedomdiagnosticstesting.com · checked Oct 7, 2026.

Compound reference: TB-500 (thymosin beta-4)

Also called
TB-500; thymosin beta-4 fragment Ac-LKKTETQ; thymosin β4 (Tβ4)
Sequence
Ac-LKKTETQ (7 residues)
Formula
C38H68N10O14
Mol. weight
889.0 g/mol
CAS
885340-08-9
Source
PubChem CID 62707662 ↗

"TB-500" is used both for the 7-residue fragment Ac-LKKTETQ (PubChem's TB-500 record, shown here) and, on many vendor COAs, for full-length thymosin beta-4 (43 residues, PubChem CID 45382195). Check which molecule the identity line on a lot's COA names. Reference data only; it describes the molecule, not any use of it. For laboratory research use only.

TB-500 (thymosin beta-4) research profile · Where to get lab-tested TB-500 (thymosin beta-4)

TB-500 Vial 10 mg lot history at Peptira

All 5 certificates we have for this product from Peptira, newest first. The chart plots HPLC purity by report date (lots without a purity figure are left out). This lot is highlighted.

95%96%97%98%99%100%98%TB5-10-001: 99.863% (Jul 16, 2025)TB5-1-002: 99.937% (Aug 13, 2025)TB5-10-003: 99.444% (Nov 20, 2025)TB5-10-004: 99.295% (Dec 18, 2025)TB5-10-005: 99.25% (Apr 16, 2026)Jul 16, 2025Apr 16, 2026
LotReport dateLabHPLC purity
TB5-10-005Apr 16, 2026Freedom Diagnostics99.25%
TB5-10-004Dec 18, 2025Freedom Diagnostics99.30%
TB5-10-003Nov 20, 2025Freedom Diagnostics99.44%
TB5-1-002Aug 13, 2025Janoshik99.94%
TB5-10-001Jul 16, 2025Janoshik99.86%

Also available from Paramount Peptides

TB-500 (thymosin beta-4) is also carried by Paramount Peptides, our other tested supplier. These are the most recent TB-500 (thymosin beta-4) certificates we have from them; each links to its own lot page.

ProductLotReport dateLabHPLC purity
Thymosin Beta 4 (TB500) (10mg)26VTBC164Jul 8, 2026Accumark Labs99.12%
Thymosin Beta 4 (TB500) (10mg)TB10-12222025-JJul 6, 2026Accumark Labs99.81%
Thymosin Beta 4 (TB500) (20mg)26VTBC110May 15, 2026Accumark Labs99.39%
Thymosin Beta 4 (TB500) (20mg)26VTBL068May 7, 2026Accumark Labs99.62%
Thymosin Beta 4 (TB500) (20mg)26VTBLO68Apr 17, 2026Janoshik99.46%

Where to get lab-tested TB-500 (thymosin beta-4): products, sizes and latest COAs →

What to check on your vial

How to check this COA in under a minute

  1. Find the lot number on your vial label and confirm it reads TB5-10-005.
  2. Open Freedom Diagnostics's record with the button above, or go to freedomdiagnosticstesting.com and enter Pept2604150109.
  3. Compare the lot number, test date, purity and identity result with the table above. They should be identical.
  4. New to COAs? Read How to read a peptide COA for what HPLC purity and mass spec identity mean.

Questions about lot TB5-10-005

What HPLC purity did TB-500 Vial 10 mg lot TB5-10-005 test at?

Freedom Diagnostics reported 99.25% HPLC purity for lot TB5-10-005 in a report dated Apr 16, 2026.

How do I verify the COA for lot TB5-10-005?

Go to freedomdiagnosticstesting.com and enter the search code Pept2604150109. The lab's own record should show lot TB5-10-005 with the same dates and results as this page.

Is lot TB5-10-005 the newest TB-500 Vial 10 mg lot from Peptira?

It's the most recent TB-500 Vial 10 mg certificate we have on file from Peptira as of Oct 8, 2026. Vendors can ship a newer lot before its certificate is posted, so check your vial label.

Does this certificate apply to my vial?

Only if the lot number printed on your vial reads TB5-10-005 exactly. A certificate of analysis covers one lot; it says nothing about other lots of the same product.

Other lots and COAs

TB-500 Vial 10 mg at Peptira

Lab-tested lot TB5-10-005 · 99.25% HPLC purity · Freedom Diagnostics

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Affiliate disclosure: We earn a commission if you buy through the vendor links on this page, at no extra cost to you. Peptides & Coffee is an independent COA reference and is not owned by or affiliated with the vendors or labs listed. Lab results are published by the vendors and labs; we link to each lab's own verification record so you can check them yourself. Stock and lots change: the vendor may be shipping a newer lot than the one on this page, so check your vial label.